Journal of visualized experiments : JoVEGenetic VectorsGenetic EngineeringChromosomesArtificialBacterial
Recombineering-based Manufacturing of Engineered Viral Vectors for Research and Therapy.
Annagiulia Scognamiglio, Simone Totaro, Alfredo Cipriano, Alfredo Nicosia, Nicola Zambrano, Emanuele Sasso, Guendalina Froechlich
Published: 202510.3791/68988
Abstract
The increasing demand for engineered viral vectors in both basic and translational research has underscored the need for flexible, rapid, and scalable methods to generate recombinant DNA viruses. Strategies relying on restriction enzyme digestion and…
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