Journal of visualized experiments : JoVEMicroRNAsHumansOligonucleotidesIn Situ HybridizationNucleic Acid Amplification Techniques
MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as A Novel Detection and Quantification Method.
Basant T Gamal, Danielle L Stolley, Christopher D Pacheco, Akshay Basi, Anna K Casasent, Jared K Burks, Sammy Ferri-Borgogno
Published: 202510.3791/68617
Abstract
When using traditional probe-based in situ hybridization (ISH) imaging detection methods, microRNAs (miRNAs) are difficult to capture due to their size, which ranges from 19 to 25 nucleotides. However, as miRNAs are key epigenetic regulators that con…
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