Structural and Spectroscopic Study of Benzoperimidines Derived from 1-Aminoanthraquinone and Their Application to Bioimaging.

In this research, we studied the synthesis and characterization of a novel amidine derivative of benzoperimidine derived from 1-aminoanthraquinone, focusing on its emission properties and potential applications in confocal laser scanning microscopy. The synthesized compound exhibited pronounced solvatochromic behavior in various solvents. Spectroscopic analysis, including 1H-, 13C-, and mass spectrometry, confirmed the chemical structure. The structure of three compounds was also determined using X-ray diffraction analysis; this study revealed the structural features of these substances in the solid state. The compound's antimicrobial activity was evaluated using the agar diffusion method with the bacterium Bacillus subtilis subsp. Spizizenii. Furthermore, the study introduces a dye designed for imaging of the parasitic flatworm Opisthorchis felineus, demonstrating its potential in visualizing biological specimens.