Multitarget Acaricidal Activity of Essential Oils from Alpinia zerumbet and Mesosphaerum suaveolens Against Tetranychus urticae.
Herus Pablo Firmino Martins, Giovana Lopes da Silva, Erika Pereira da Silva, Mariano Oscar Anibal Ibanez Rojas, Francisco José Teixeira Gonçalves, Izaias Santos Marques, Marcos Vinícius de Sousa Negreiros, Victório Alessandro de Leão Loeschke Morais, Franciele Costa de Sousa, Antonio José Cantanhede Filho, Bruno de Araújo Gomes, Edson Rodrigues Filho, Solange Maria de França, Adalberto Hipólito de Sousa, Lucas Martins Lopes
Abstract
Open AccessThe two-spotted spider mite, Tetranychus urticae, is a global pest with increasing resistance to conventional acaricides, prompting the search for sustainable alternatives. Essential oils (EOs) are promising botanical biocides due to ecological safety and multitarget action. We evaluated lethal and sublethal effects of EOs from Alpinia zerumbet and Mesosphaerum suaveolens against T. urticae. Oils were obtained by hydrodistillation and characterized by GC-MS (major constituents: A. zerumbet-1,8-cineole 14.05%, sabinene 12.6%; M. suaveolens-β-sabinene (predominant), spathulenol 12.28%, 1,8-cineole 11.01%). In adult bioassays, M. suaveolens was more toxic (LC50 = 4.24 µL mL-1), whereas A. zerumbet showed LC50 = 8.74 and LC90 = 46.24 µL mL-1. In ovicidal assays at LC90, egg viability declined to 2% with A. zerumbet versus 57% with M. suaveolens. Repellency at sublethal concentrations (LC20-LC30) was high for both oils (≥75%) and exceeded 90% for M. suaveolens. Both oils suppressed population growth (instantaneous rate ri reduced from 0.5848 in the control to 0.4746-0.5155 under treatments). PCA confirmed lethal concentration and repellency as the main discriminators among treatments. These data demonstrate the multitarget potential of A. zerumbet and M. suaveolens EOs as botanical acaricides for sustainable management of T. urticae.