Implementation of Instrumental Analytical Methods, Image Analysis and Chemometrics for the Comparative Evaluation of Citrus Fruit Peels.

Citrus fruit cultivation and processing are constantly rising due to the increasing market demand and diverse utilization potentials. This generates large quantities of residues, predominantly composed of citrus peels. This study aimed to evaluate six different citrus peels using rapid and/or nondestructive instrumental analytical techniques such as ATR-FTIR spectroscopy, spectrophotometric assays, image textural analysis and physicochemical parameter determination. Image textural features managed to discriminate citrus peels based on their structure uniformity, which was found increased in lemon (C. limon) and yellow grapefruit (C. paradisi), whereas clementine (C. clementina) and red grapefruit (C. paradisi) images exhibited an increased non-uniformity of the structure. Physicochemical parameters provided insights into the quality characteristics of citrus peels, while their high ascorbic acid content seems to enhance their antioxidant activity. The obtained results from phenolic and flavonoid content determination indicated a high concentration of polyphenols in the peels, which is aligned with the ATR-FTIR spectra absorption bands. Furthermore, the spectrophotometric assays' strong correlation suggests that the antioxidant activity of citrus peels is mainly attributed to polyphenols. Ultimately, a chemometric model was employed to provide a comprehensive understanding of the analytical methods' interactions. Hence, citrus peels' significant biochemical and, consequently, economic value can be highlighted, underscoring the importance of further research.