Association of serum cyclooxygenase-2 levels with hand-foot syndrome in patients receiving capecitabine: an exploratory analysis of D-TORCH study.

Background: The D-TORCH trial demonstrated superiority of 1% topical diclofenac over placebo in preventing capecitabine-induced hand-foot syndrome (HFS). We conducted an exploratory analysis of this study to assess the relationship between HFS and serum levels of the inflammatory marker, cyclooxygenase-2 (COX-2). Methods: Serum COX-2 levels were measured in patients in the D-TORCH study's experimental and placebo arms at baseline and 12 weeks of capecitabine-based therapy or at the development of HFS (whichever occurred earlier) and in 20 age-matched healthy controls using a human COX-2 ELISA kit (E-EL-H5574). Results: 233 (88.5%) patients of the D-TORCH cohort (n = 263) underwent serial COX-2 analysis. The population was female predominant (n = 165, 70.8) with a median age of 47 years (range: 19-73), including breast (n = 130, 55.8%) and gastrointestinal cancers (n = 103, 44.2%). 31 (13.3%) patients developed any-grade HFS, with 25 (10.7%) having grade 2 or worse HFS. Mean serum COX-2 levels at baseline and 12 weeks did not show a statistically significant difference (mean + standard deviation, 3.41 + 2.15 ng/ml versus 3.35 + 2.40 ng/ml, p = 0.69); however, baseline levels in patients were significantly higher than healthy controls (p < 0.001). No statistically significant difference was found between serial COX-2 levels by gender, use of topical diclofenac, type of malignancy or severity of HFS. Conclusion: Serum COX-2 levels did not show a significant change with capecitabine-based therapy, regardless of the use of topical diclofenac possibly reflecting the predominant stromal production of the enzyme. This finding highlights the need to assess HFS-affected tissues for local COX-2 immuno-expression along with further blood-based biomarkers.