An updated multigene phylogeny of Leotiomycetes.

A paper by Johnston and others published in 2019 provided three Leotiomycetes-wide phylogenies, one based on genomes, one a multigene phylogeny with up to 15 genes, and one based on ITS sequences. The genomic and multigene phylogenies provided a backbone phylogeny for the class that has proven to be robust, providing a phylogenetically stable concept for the large order Helotiales. However, a lack of genomic data for the clades outside of Helotiales, towards the root of the tree, meant that relationships in this part of the tree remained somewhat poorly resolved. The ITS phylogeny, although phylogenetically much less informative, provided a wider taxon coverage, focussing on the type species of genera not otherwise treated for which ITS data was available. This paper treats genera listed as Leotiomycetes in the Outline of Fungi 2024. It incorporates newly available DNA sequence data and taxonomic changes, especially for genera, families, and orders published since 2019, into the 2019 multigene and ITS analyses. The genomic phylogeny is not updated. Citation: Johnston PR, Park D (2025). An updated multigene phylogeny of Leotiomycetes. Persoonia 55: 527-566. doi: 10.3114/persoonia.2025.55.13.