Impact of platelet-rich plasma at different concentrations on genes of apoptotic pathway and differentiation of stem cells in cyclophosphamide-induced ovarian failure in a mouse model: An experimental study.

Background: Platelet-rich plasma (PRP), a new therapeutic technique, has the potential to regenerate failing ovarian tissue and alleviate symptoms in patients with premature ovarian failure (POF). Objective: This study examined the effect of different platelet concentrations on ovarian function, investigated the apoptosis genes B-cell lymphoma 2 (BCL-2) and Bcl-2-associated X protein, and the differentiation gene octamer-binding transcription factor 4. Materials and Methods: In this experimental study, 30 female Syrian mice (8-10 wk, 25-30 gr) were randomly divided into 5 groups (n = 6/each): 1) normal control, 2) POF + phosphate buffered saline, 3) POF + PRP 0.25 ml/kg, 4) POF + PRP 0.5 ml/kg, and 5) POF + PRP 1 ml/kg. The cyclophosphamide was used to create a mouse model of POF. After 2 wk from injection of PRP and phosphate buffered saline, ovaries were removed for histological and molecular analysis. Results: The distribution of different follicle types in the POF + PRP group was the same as that of the control group, according to a morphometric study. Bcl-2-associated X protein gene expression was significantly reduced in the groups that received PRP (p < 0.001). BCL-2 and octamer-binding transcription factor 4 gene expression was also increased in the PRP groups (p < 0.05), but the gene expression was different in different platelet concentrations. Conclusion: The results showed that PRP can be effective in POF, but different dosages of PRP can have different effects on the recovery of ovarian.