Investigation of the Combined Effects of Propylparaben and Methylparaben on Biochemical and Histological Parameters in Male Rats.

Objective: This research aimed to study the effects of co-exposure to methylparaben and propylparaben (MeP+PrP) on renal and hepatic parameters in male rats. Materials and Methods: MeP and PrP were mixed at a ratio of 1:1. MeP+PrP doses were administered to 42-day-old male rats on a daily basis for 30 days. Each of the five groups consisted of six male rats, including three MeP+PrP dose groups with daily combination doses of 10 mg/kg bw, 100 mg/kg bw, and 500 mg/kg bw. The other two groups included the positive control group exposed to a daily dose of 50 mg/kg bw Bisphenol-A (BPA), and the corn oil control group used as a vehicle. At the end of the procedure period, liver and kidney tissues were obtained from histopathological analysis, morphometric analysis was performed on kidney tissue, and hematological and biochemical parameters were examined. Results: Histopathological changes, such as tubular degeneration, edema, fibrous tissue formation, and congestion, were observed in the kidney tissue, while degeneration, edema, and congestion were observed in the liver tissue. Statistical analysis comparing the administered groups to the corn oil control group revealed significant variations in biochemistry and hematological markers. Conclusion: These findings demonstrate that co-exposure to MeP and PrP alters biochemical and hematological parameters and negatively affects the liver and kidney tissues of male rats.