UPLC-MS/MS analysis and chemical profile of Ficus pertusa L . bark, an ethnomedicinal resource from intertropical America.

Background: Ficus pertusa is a native species from intertropical America used for medicinal purpouses. However, despite its importance as ethnobotanical resource, there is poor chemical information about its extracts. Therefore, the objective of this study was to evaluate the phytochemical profile of the hydroalcoholic extract of F. pertusa barks through UPLC-MS/MS, as well as its phenolic and flavonoid content. Methodology: The chemical identification was performed by Ultra-High Liquid Chromatography coupled to Mass Spectrometry, whereas the Total Phenolic content and Total Flavonoid quantification were measured through UV spectrometry. Results: As a result, 19 substances were identified, which are mostly included within the group of condensed tannins, phenolic acids and flavonoids, such as procyanidins, chlorogenic acid and luteolin. Conclusion: The identification of the main substances of F. pertusa's bark and the measurement of its polyphenols content can guide the understanding of the pharmacological mechanisms involved in its ethnomedicinal uses.