Microbial cell factoriesSingle-Chain AntibodiesEpithelial Cell Adhesion MoleculeEscherichia coli ProteinsEscherichia coliAcetates
Enhanced anti-EpCAM ScFv production and reduced acetate overflow in a BW25113-derived E. coli strain with ArcA and Pka deletions.
Majid Basafa, Aidin Behravan, Hossein Vahidi, Atieh Hashemi
Published: 202510.1186/s12934-025-02907-6
Abstract
Escherichia coli strains are widely utilized as cell factories for recombinant protein production. However, acetate overflow remains a significant challenge that negatively impacts both biomass yield and protein expression. Here, we evaluated a previ…
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