Advances in high-throughput mutation breeding systems integrating atmospheric and room-temperature plasma (ARTP) with droplet-based microfluidics.

In recent years, the integration of atmospheric and room-temperature plasma (ARTP) mutagenesis with droplet-based microfluidic (DBMF) technology has enabled the development of a novel high-efficiency mutagenesis and screening system. This system not only enhances microbial mutagenesis efficiency but also achieves precise screening and high-throughput detection, demonstrating broad applications in biosynthesis, fermentation engineering, biological feed production, edible fungus breeding, and environmental remediation. This review comprehensively elaborates on the principles and advantages of the system and discusses its diverse applications across multiple fields.