The CRISPR journal
Streptococcus uberis Cas9-A Compact Type II-A Nuclease Recognizing a Unique PAM and Functional in Human Cells.
Aleksandra Vasileva, Marina Abramova, Polina Selkova, Anatolii Arseniev, Olga Musharova, Polina Malysheva, Alina Demkina, Mikhail Khodorkovskii, Konstantin Severinov
Published: 202510.1177/25731599251404417
Abstract
Several type II Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas9 RNA-guided nucleases are commonly used for genome engineering. Their relatively large size and requirements for specific protospacer adjacent motif (PAM) sequence…
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