American journal of physiology. Cell physiologyAnimalsOxygenOxygen ConsumptionRatsRetinal Pigment Epithelium
Incorporation of gas/liquid equilibration control into a perifusion system to measure tissue-specific O2 concentration dependency of O2 consumption rate.
Varun Kamat, Khang Bao, Matthew K Grumbine, Lui Tsumura, James C Hermanson, John Kramlich, Laura Pyle, Rayne Lim, Jennifer R Chao, Daniel Hass, James B Hurley, Ian R Sweet
Published: 202510.1152/ajpcell.00640.2025
Abstract
Oxygen (O2) regulates a multitude of cell functions, and many pathological states are linked to its delivery. We present an automated system for implementing rapid changes in dissolved gas composition in the inflow of a perifusion system that facilit…
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