Journal of clinical microbiology
Challenges in accurate HDV RNA quantification: inter-assay variability and the impact of thermal shock.
Felipe Pérez-García, Ana Virseda-Berdices, Carlos Pita-Martínez, Mario Muñoz Monte, Daniel Sepúlveda-Crespo, Helena Codina, Roberto Alonso, Lara Mesones, Sandra Rodrigo, Juan Macías, Luis Miguel Real, Juan Cuadros-González, Isidoro Martínez, Salvador Resino
Published: 202510.1128/jcm.01517-25
Abstract
Quantitative RT-PCR (qRT-PCR) is essential for monitoring hepatitis delta virus (HDV) RNA, yet assays lack standardization. We aimed to evaluate the performance of three qRT-PCR assays and to assess the impact of a pre-analytical thermal shock proced…
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