The New phytologist
Iterative pathway engineering of the diatom Phaeodactylum tricornutum to enhance the biosynthesis of long-chain polyunsaturated fatty acids using the Cre recombinase-mediated marker recycling.
Junkai Zhu, Weizhong Chen, Shuangqing Li, Andrew E Allen, Xinde Xu, Xiaoping Wang, Haibo Jiang, Yangmin Gong
Published: 202510.1111/nph.70829
Abstract
In the diatom Phaeodactylum tricornutum there are limited selectable markers available for genetic manipulation, which has hampered the biotechnological application of metabolic engineering in this algal species. Here, we developed a bacteriophage P1…
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