Journal of integrative plant biology
A surrogate-selection-enhanced TnpBmax platform enables efficient genome editing in rice and tomato.
Enze Li, Jiyong Xie, Xinbo Li, Zai Zheng, Mugui Wang, Jian-Kang Zhu, Yifu Tian
Published: 202610.1111/jipb.70159
Abstract
TnpB, a compact RNA-guided nuclease ancestral to Cas12, is optimized for plant genome editing. A 99-nt enhanced RNA and T5 exonuclease fusion generate TnpBe5, boosting rice editing 2.5-fold. Coupling with a single-strands annealing (SSA)-responsive H…
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