Journal of integrative plant biology
Interspecies rice versus Arabidopsis thaliana protein-protein interactome profiling by touch-down overlapping PCR coupled with HiFi long-read sequencing.
Jie Huang, Yu Cheng, Jing Ruan, Xixi Liu, Dandan Xia, Yiting Chen, Delong Fan, Jiezheng Ying, Yifeng Wang, Xiaohong Tong, Zhiyong Li, Dawei Xue, Jianwei Zhang, Jian Zhang, Yuxuan Hou
Published: 202510.1111/jipb.70107
Abstract
Touch-down overlapping PCR coupled with HiFi long-read sequencing, a high-throughput method for large-scale profiling of protein-protein interactions based on stitch-PCR identified 7,726 high-confidence interactions between rice and Arabidopsis prote…
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