Insect science
Knockout of Ligase4 gene enhances homology-directed repair efficiency in Plutella xylostella.
Faisal Munir, Uroosa Zaheer, Sheng Lin, Anam Noreen Abbas, Mubashir Tariq, Jianying Liao, Qingxuan Qiao, Yanpeng Chang, Yussuf Mohamed Salum, Ibrahim Adams, Hui Wei, Muhammad Asad, Guang Yang
Published: 202510.1111/1744-7917.70184
Abstract
Knock-in efficiency via CRISPR/Cas9-based homology-directed repair (HDR) is low in lepidopteran insects due to predominance of knockout through non-homologous end joining (NHEJ) pathway. DNA ligase 4 (LIG4), a core protein of NHEJ complex and double-…
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