Extra-ciliary role for polycystins in regulation of Ezrin and renal tubular morphology.

Full understanding of the functions of the polycystin proteins, PC1 and PC2, in renal epithelial cells is obscured by signaling complexity and renal injury that occurs in Autosomal Dominant Polycystic Kidney Disease (ADPKD). The polycystins likely function as a complex in the primary cilium, yet previous work hinted at a critical role for PC1 function outside of the primary cilium (extra-ciliary) during tubule development. Here, we investigate an extra-ciliary role for the polycystins in regulating renal cell and tubular morphology. First, we found acute loss of polycystins significantly increased the circularity of renal epithelial cells and tubuloids grown in 3D culture. Next, we demonstrated that both PC1 and PC2 can immunoprecipitate Ezrin, an ERM protein important for apical compartment shape. In human ADPKD renal cystic tissue, and after acute inducible knockout of Pkd1 or Pkd2, we found that Ezrin protein abundance is significantly reduced, with the remaining Ezrin protein mis-localized. Immunofluorescence in 2D cells and 3D tubuloids suggested acute polycystin loss specifically reduced the active form of Ezrin at the apical surface, leaving inactive Ezrin colocalized with ZO1 in the cell junctions. A specific ERM phosphorylation inhibitor, NSC668394, phenocopied the increased circularity observed in the Pkd1 knockout spheroids, as did inhibition of PKC activity, implicating the polycystin complex in regulating Ezrin phosphorylation. Our data strongly support a role for the polycystin complex in regulating renal cell and tubular shape via interactions with the ERM protein Ezrin, interactions that do not require trafficking to the primary cilium.