Novel key genes in the pathogenesis of recurrent pelvic organ prolapse identified via bioinformatics analysis.

The causes of pelvic organ prolapse (POP) recurrence are sufficiently understood. Few studies have investigated the key genes of the recurrence of POP. In the present study, we screened the hub genes responsible for the recurrence of POP. The GSE28660 gene expression dataset contained microarray data of 4 recurrent POP and 4 primary POP uterosacral ligaments. We used the online gene expression omnibus microarray expression profiling dataset to identify differentially expressed genes. Further analyses of functional enrichment and protein-protein interaction networks (PPIs) were conducted, and key modules were identified. In the next step, we used the CIBERSORT algorithm to investigate differences in immune cell infiltration between recurrent and primary POP tissues. A total of 84 upregulated genes and 32 downregulated genes were identified. DNA microarray analysis of the human genome identified 116 genes associated with recurrence POP, and 2 hub genes, including cell death-inducing DFFA-like effector (CIDEA) and hemoglobin subunit delta (HBD), may contribute to the pathogenesis of recurrence POP, potentially providing diagnostic and therapeutic value.