Nucleic acids researchCRISPR-Cas SystemsDNA CleavageDeoxyribonuclease ICRISPR-Associated ProteinsCatalytic Domain
Conformational dynamics of CRISPR-Cas type I-F-HNH inform nickase engineering in a cascade scaffold.
Anders Fuglsang, Sweta Suman Rout, Eliska Bartl Koutna, Nicholas Sofos, Alejandro Redondo Gallego, Guillermo Montoya
Published: 202610.1093/nar/gkag053
Abstract
The type I-FHNH CRISPR-Cas system is a non-canonical Class 1 effector complex distinguished by the replacement of the Cas3 recruitment domain with a catalytic HNH domain in Cas8, enabling autonomous DNA cleavage without accessory nucleases. Using cry…
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