Letters in applied microbiologyMycoplasma genitaliumGenomeBacterialHumansPolymerase Chain Reaction
Sequencing of Mycoplasma genitalium genomes using a tiling amplicon method on the Nanopore MinION.
Teck-Phui Chua, Jennifer A Danielewski, Jose L Huaman, Erica L Plummer, Catriona S Bradshaw, Lenka A Vodstrcil, Dorothy A Machalek, Suzanne M Garland, Gerald L Murray
Published: 202510.1093/lambio/ovaf119
Abstract
Mycoplasma genitalium is challenging to work with and new methods are needed to study this bacterium directly in clinical samples. This study designed and validated a proof-of-concept polymerase chain reaction (PCR)-based 'tiling' methodology to sequ…
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