Methods and applications in fluorescenceMorpholinesBuffers2-AminopurinePiperazinesHydrogen-Ion Concentration
Mechanistic basis for 2-aminopurine quenching by morpholine- and piperazine-based Good's buffers.
Souvik Poddar, Benjamin Glennon, Marcia Levitus
Published: 202610.1088/2050-6120/ae35d0
Abstract
2-Aminopurine (2AP) is the most widely used fluorescent nucleobase analog in DNA and RNA research. While quenching of 2AP by DNA bases has been extensively characterized, the effect of extrinsic quenchers has received far less attention. This study e…
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