Characterization of the complete chloroplast genome of Anemone sylvestris L. 1753 (Ranunculaceae).

Anemone sylvestris Linnaeus (1753) is a species in the Ranunculaceae family within the genus Anemone. It is mainly distributed in China, Croatia, and Poland. The chloroplast genome has a total length of 161,033 bp, comprising a large single-copy region of 81,144 bp, a small single-copy region of 17,393 bp, and two inverted repeat regions of 31,248 bp each. The GC content is 37.54%. A total of 134 genes were annotated, including 90 protein-coding genes, 8 rRNA genes, and 36 tRNA genes. Phylogenetic analysis using the maximum likelihood method robustly supported the close relationship of A. sylvestris with other Anemone species, namely A. raddeana, A. reflexa, and A. altaica, forming a well-defined monophyletic clade. The complete chloroplast genome of A. sylvestris reported here provides a useful resource for subsequent phylogenetic, identification, and resource related studies.