Stiripentol-loaded self-nanoemulsifying delivery system for glioblastoma: therapeutic efficacy in 3D spheroid and xenograft model.

AIM: To develop, formulate, and evaluate stiripentol (STP)-loaded Self-Nano Emulsifying Drug Delivery System (SNEDDS) for its potential as a Glioblastoma (GBM) therapeutic. MATERIALS AND METHODS: Characterization of STP SNEDDS using polydispersity index, particle size, zeta potential, and pH stability. Efficacy testing using BBB permeation, 3D spheroid, tumor xenograft, immunoblotting, immunohistochemistry, stability, and toxicity assessment. RESULTS: STP SNEDDS showed improved water solubility and drug release profile, stability, relative safety in normal cells and brain tissue, and decreased 3D spheroid growth, tumor volume, tumor weight, and proliferative marker Ki67. CONCLUSION: This work highlights the potential of STP SNEDDS as a potential therapeutic for GBM.