Expert review of molecular diagnosticsCRISPR-Cas SystemsHumansGene EditingRNAGuide
Guarding the genome: strategies for off-target detection and minimization in CRISPR/Cas9 system.
Malihe Lotfi, Zahra Farshchian Yazdi, Mohammad Reza Hashemi, Mohammad Reza Abbaszadegan, Samaneh Sharif
Published: 202510.1080/14737159.2025.2581677
Abstract
INTRODUCTION: CRISPR-based genome editing has revolutionized molecular diagnostics and therapeutic applications, offering precise genetic modifications. However, off-target effects remain a significant challenge, potentially leading to unintended mut…
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