Enhanced sensitivity of lateral flow assay for NGAL detection using cysteamine-modified gold nanoparticles.
Paweena Tunakhun, Sawinee Ngernpimai, Patcharaporn Tippayawat, Kiattawee Choowongkomon, Sirirat Anutrakulchai, Nicha Charoensri, Ratree Tavichakorntrakool, Sakda Daduang, Oranee Srichaiyapol, Pornsuda Maraming, Jeerati Prompipak, Patcharee Boonsiri, Jureerut Daduang
Abstract
Open AccessNeutrophil gelatinase-associated lipocalin (NGAL) has emerged as a crucial biomarker for various clinical conditions, including acute kidney injury. However, enhancing the sensitivity of the lateral flow strip (LFS) for NGAL detection remains a challenge. LFS has revolutionized point-of-care diagnostics, offering rapid and cost-effective detection of biomarkers. This study explores the potential of cysteamine gold nanoparticles (AuNPs) as a novel conjugate for NGAL detection in LFS, comparing their sensitivity enhancement against traditional citrate-coated AuNPs (Cit-AuNPs). Through comprehensive experimentation and analysis, we demonstrate the superior performance of cysteamine-coated AuNPs (Cys-AuNPs) LFS in detecting NGAL, paving the way for more sensitive point-of-care diagnostics in relevant clinical settings.