The Analyst
Exploiting Pfu DNA polymerase's differential bypass capacity for AP vs. dU sites: a novel strategy for sensitive uracil-DNA glycosylase detection.
Run-Hong Zhang, Chen-Yu Xue, Jia-Hui Dong, Xu-Yang Shen, Yi-Xuan Gu, Ying Liu, Ying-Lin Zhou, Xin-Xiang Zhang
Published: 202610.1039/d5an01082a
Abstract
In biological systems, uracil repair in DNA is initiated by uracil-DNA glycosylase (UDG), which removes uracil bases from their corresponding sites to produce abasic sites (AP sites). This enzyme is conserved and functionally critical across living o…
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