The AnalystHumansBiosensing TechniquesAlkaline PhosphataseHeLa CellsCRISPR-Cas Systems
TdT/Cas12a cascade amplification biosensor for sensitive ALP activity detection.
Chengbin Pei, Bei Yan, Yan Wang, Ting Chen, Kunhao Du, Lianghong Ma, Juan Wang
Published: 202510.1039/d5an00938c
Abstract
This study presents a novel biosensor based on TdT and CRISPR-Cas12a, which integrates the catalytic activity of terminal deoxynucleotidyl transferase (TdT) with the trans-cleavage property of CRISPR-Cas12a to achieve ultra-sensitive biomolecular det…
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