The EMBO journal
Bacterial ubiquitin ligase engineered for small molecule and protein target identification.
James S Ye, Abir Majumdar, Brenden C Park, Miles H Black, Ting-Sung Hsieh, Adam Osinski, Kelly A Servage, Kartik Kulkarni, Jacinth Naidoo, Neal M Alto, Margaret M Stratton, Dominique Alfandari, Joseph M Ready, Krzysztof Pawłowski, Diana R Tomchick
Published: 202610.1038/s44318-025-00665-0
Abstract
The Legionella SidE effectors ubiquitinate host proteins independently of the canonical E1-E2 cascade. Here we engineer the SidE ligases to develop a modular proximity ligation approach for the identification of targets of small molecules and protein…
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