A High-quality chromosome-level genome assembly of Swertia przewalskii Pissjauk.

Swertia przewalskii Pissjauk. belonging to Swertia (subtribe Swertiinae, Gentianaceae), is one of the original plant sources of the Tibetan medicine "Zangyinchen" has been included in the initial list of plant species with extremely small populations in Qinghai province. To gain deeper insights into the threats to S. przewalskii survival, we assembled a high-quality, chromosome-level genome using a combination of Illumina short-read, PacBio HiFi long-read, and Hi-C scaffolding technologies. The assembled genome has a size of ~2027.49 Mb, and demonstrates excellent continuity, consistency, and completeness (contig N50 = 2,569,777, mapping rate = 98.87%, coverage = 99.29%, BUSCO = 96.9%). The assembled sequences could be anchored to twelve chromosomes. Additionally, genome annotation revealed 35,701 protein-coding genes, 289 microRNAs (miRNAs), 1,039 transfer RNAs (tRNAs), 3,681 ribosomal RNAs (rRNAs). 32,775 genes were functionally annotated using several databases, including NR (31,587, 88.48%), GO (23,883, 66.90%), KOG (663, 1.86%), and Pfam (22,995, 64.41%), KEGG (11,964, 33.51%). This study reports a high-quality, chromosome-level genome assembly of S. przewalskii, providing a foundational resource for its conservation biology.