A near-complete reassembled haplotype-resolved reference genome of Acer truncatum.

The genus Acer (maples) is widely distributed and holds significant ecological and economic value, with over 200 species, including Acer truncatum, an important member known for its ornamental features, environmental stress tolerance, and high content of unsaturated fatty acids and nervonic acid in its seeds. Despite the genus's importance, a high-completeness genome assembly has been lacking. In this study, we present a near-complete telomere-to-telomere (T2T) genome assembly of A. truncatum using advanced sequencing technologies, including PacBio HiFi long reads, ONT ultra-long reads, and Hi-C chromatin conformation capture. The resulting 1.2 Gb haplotype-resolved genome contains 58,569 protein-coding genes and shows a 4.8-fold improvement in contiguity over previous assemblies, with a scaffold N50 of 44 Mb. This high-quality genome provides a foundational resource for investigating the evolutionary history, functional genomics, and molecular breeding of A. truncatum.