Chromosome-Level Genome Assembly of Donacia provosti.

The evolutionary adaptation of insects to aquatic environments remains a fascinating study area in genomics and evolutionary biology. Here, we present a high-quality chromosome-level reference genome of Donacia provosti (Coleoptera: Chrysomelidae), an ecologically and agriculturally significant aquatic pest species, using a combination of PacBio HiFi, Illunima and Hi-C sequencing technologies. The assembly comprises 15 chromosomes, totaling 1.76 Gb in size, and exhibit high contiguity, with contig and scaffold N50 values of 27 Mb and 71 Mb, respectively. Genome completeness, as assessed by BUSCO, was 98.6%. A total of 1.35 Gb of repeat sequences and 20,130 protein-coding genes were identified in this genome. Moreover, several gene families associated with vision and immune are expanded significantly in this pest. This high-quality D. provosti genome assembly would provide a valuable resource for future investigations into its ecological adaptations and the development of pest control measures.