Telomere-to-telomere gapless genome assembly of Acorus tatarinowii.

Acorus tatarinowii, a medicinal herb belonging to the Acoraceae family, is widely used in aromatherapy and cognitive enhancement due to its rich repertoire of over 160 natural products. Phylogenetically, it holds a key position as the sister lineage to all other monocots, underscoring the need for a high-quality genome assembly to facilitate in-depth evolutionary and functional studies. In this study, we present a telomere-to-telomere (T2T) genome assembly of A. tatarinowii, spanning 359.36 Mb with a scaffold N50 of 32.54 Mb. The assembly demonstrates reference-grade quality, supported by a consensus quality value (QV) of 45.41 and a long terminal repeat assembly index (LAI) of 10.04. BUSCO analysis of the genome revealed 97.6% complete alignments, confirming the assembly's high continuity and completeness. Genome annotation identified 24,879 protein-coding genes, with repetitive sequences comprising 57.51% of the genome. BUSCO assessment of gene models further validated 97.2% completeness. This high-quality T2T genome assembly not only elucidates the genomic features of this early-diverging monocot species but also provides a robust foundation for future functional genomics research.