Nature structural & molecular biology
An anti-CRISPR targets the sgRNA to block Cas9 and guides the design of enhanced genome editors.
Ling Yu, Mingyu Yin, Yuwei Zhu, Zebin Lu, Bao Xiao, Fengxia Zhou, Yue Yu, Zhiwei Huang
Published: 202610.1038/s41594-025-01741-z
Abstract
Bacteriophages have evolved anti-CRISPR (Acr) proteins to combat the adaptive immunity provided by bacterial CRISPR-Cas systems. Here, we report the cryo-electron microscopy structure of an anti-Cas9 protein AcrIIA27 bound to SpyCas9-sgRNA (single gu…
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