Nature biotechnology
A parts list of promoters and gRNA scaffolds for mammalian genome engineering and molecular recording.
Troy A McDiarmid, Megan L Taylor, Wei Chen, Florence M Chardon, Junhong Choi, Hanna Liao, Xiaoyi Li, Haedong Kim, Jean-Benoît Lalanne, Tony Li, Jenny F Nathans, Beth K Martin, Jordan Knuth, Alessandro L V Coradini, Jesse M Gray
Published: 202510.1038/s41587-025-02896-2
Abstract
A standardized 'parts list' of sequences for genetic engineering of microbes has been indispensable to progress in synthetic biology, but few analogous parts exist for mammalian systems. Here we design libraries of extant, ancestral, mutagenized or m…
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