Nature communications
Af-CUT&Tag: a sensitive and antibody-free chromatin profiling method using genetically encoded tags and high-affinity binders fused to Tn5.
Xindong Wang, Xusheng Deng, Lu Qiu, Junjia Liu, Hengxiang Shen, Haoran Du, Weijie Li, Linxuan Song, Wenhui Deng, Xiaoning Dong, Yi Han, Benchao Liu, Jialiang Huang, Zengpeng Li, Yongyou Zhang
Published: 202610.1038/s41467-026-68454-9
Abstract
Conventional chromatin profiling techniques are often limited by antibody availability and performance. Here, we introduce Af-CUT&Tag, a target antibody-free method that overcomes these limitations by using CRISPR-integrated peptide tags (HiBiT/ALFA-…
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