ACS sensorsNucleic Acid Amplification TechniquesCRISPR-Cas SystemsNucleic AcidsHumansMolecular Diagnostic Techniques
One-Pot CRISPR-Based Isothermal Amplification for Nucleic Acid Detection: A Comparative Review of Different Strategies.
Cong Shi, Zhou Yu, Huimin Tan, Weilin Li, Yingjun Wang, Yun Wang, Qinghai Zhang, Yan Man
Published: 202510.1021/acssensors.5c00806
Abstract
Nucleic acid detection plays an important role in pathogen monitoring and disease diagnosis. CRISPR one-pot assays combined with isothermal amplification are emerging as promising point-of-care technologies that simplify workflows while increasing se…
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