Extensive Alanine Scanning of Loop Regions in Ketosynthase Domains Identifies Non-Active Site Mutations with Drastic Effects on Polyketide Biosynthesis.

Modular polyketide synthases (PKSs) produce diverse natural products with significant pharmaceutical value, but their protein engineering for drug discovery is hampered by the unpredictable substrate specificity of their ketosynthase (KS) domains. Wh…

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