The journal of physical chemistry. BMolecular Dynamics SimulationCRISPR-Cas SystemsDNACRISPR-Associated Protein 9Gene Editing
Design Rules for Expanding PAM Compatibility in CRISPR-Cas9 from the VQR, VRER and EQR variants.
Francisco Vieyra, Chinmai Pindi, George P Lisi, Uriel N Morzan, Giulia Palermo
Published: 202510.1021/acs.jpcb.5c06153
Abstract
Expanding the range of Protospacer Adjacent Motifs (PAMs) recognized by CRISPR-Cas9 is essential for broadening genome-editing applications. Here, we combine molecular dynamics simulations with graph-theory and centrality analyses to dissect the prin…
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