Journal of chemical theory and computationAllosteric RegulationMolecular Dynamics SimulationRNAGuideCRISPR-Cas Systems
Differential Allosteric Modulation of Cas9 Specificity.
Yuanhao Li, Xin Li, Yingjie Chen, Yanqing Wang, Zhicheng Zuo
Published: 202610.1021/acs.jctc.5c01919
Abstract
Both RNA- and protein-based strategies have been developed to mitigate off-target cleavage by CRISPR-Cas9, yielding noncanonical guide RNAs (gRNAs) and Cas9 variants with enhanced gene-editing precision. However, the molecular mechanisms by which suc…
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