Journal of agricultural and food chemistryIsomaltoseEnzyme StabilityDirected Molecular EvolutionGlucosyltransferasesBacterial Proteins
Engineering Sucrose Isomerase by Truncation and Directed Evolution for Thermostability and Efficient Isomaltulose Production.
Hao Meng, Chao Li, Qian Shen, Xi-Rong Liu, Chun-Ling Zeng, Fang Yang, Xiao-Juan Zhao, Jin-Lan Xia
Published: 202510.1021/acs.jafc.5c09800
Abstract
Isomaltulose, a functional reducing sugar with promising applications, is biosynthesized from sucrose using sucrose isomerase (SIase). Nonetheless, the practical application of SIase has been hindered by its instability and reduced activity. In this…
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