Bioconjugate chemistryHumansRNASmall InterferingGlutathioneGene Silencing
"Zip-to-Cytosol": Glutathione-Cleavable Fluorinated Polyplexes Deliver siRNA at Single-Digit Nanomolar Dose with >90% Gene Silencing.
Yue Wang, Jingyi Wang, Hongyan Cui, Liuwei Zhang, Ruohan Zhao, Zhongwang Liu, Qian Zhong, Xin Wang, Yaojie Wang, Qixian Chen, Haining Yu, Yan Zhao
Published: 202610.1021/acs.bioconjchem.5c00554
Abstract
A fluorinated, disulfide-cross-linked polyplex platform (PFND) was developed for safe and potent cytosolic delivery of siRNA. Branched PEI (25 kDa) was first perfluoro-acylated to yield a membrane-zipper PF backbone, followed by orthogonal installati…
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