Analytical chemistry
Single Cell Quantification of Let-7a with an Electrophoresis-Assisted Tandem Signal Amplification Strategy based on Intracellular Catalytic Hairpin Assembly-Branched Hybridization Chain Reaction-MNAzyme.
Yuhai Chen, Ruiyan Li, Shengyi Zhang, Caiying Li, Shulin Zhao, Yong Huang, Jingjin Zhao
Published: 202610.1021/acs.analchem.5c06513
Abstract
The microRNA let-7a plays a pivotal role in tumor suppression by regulating RAS and other oncogenes. However, due to its extremely low abundance in individual cells, quantitative analysis of let-7a at the single-cell level remains challenging. In thi…
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