Analytical chemistrySequence AnalysisRNAMass SpectrometryRibonuclease T1Base Sequence
Addressing the Determinants of Ambiguous Assignments Incurred by the Mass Spectrometry Sequence Analysis of Large Modified RNAs.
Daniele Rollo, Thomas Kenderdine, Ghazaleh Yassaghi, Cole Angell, Daniele Fabris
Published: 202610.1021/acs.analchem.5c05115
Abstract
Common mass spectrometric approaches for the characterization of large RNAs involve cleaving the initial strand into smaller sections amenable to mass mapping and gas-phase sequencing. These approaches are plagued by the significant loss of informati…
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