Analytical chemistryIsotachophoresisKineticsCRISPR-Cas Systems
Reaction Kinetics of CRISPR trans-Cleavage Controlled Using Isotachophoresis.
Qi Jiang, Ashwin Ramachandran, Alexandre S Avaro, Diego A Huyke, Juan G Santiago
Published: 202510.1021/acs.analchem.5c04301
Abstract
CRISPR-based diagnostics are powerful tools for nucleic acid detection due to their high specificity and programmability. However, assay sensitivity is often limited by the slow kinetics of the trans-cleavage reaction, which typically proceeds at a r…
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