Analytical chemistry
High-Resolution Ion Mobility Mass Spectrometry for Separation of Oligonucleotide Phosphorothioate Diastereomers.
Molly S Blevins, Jie Du, Ralph Aderorho, Raymond Lieu, Christopher M Crittenden, Tao Chen
Published: 202610.1021/acs.analchem.5c04150
Abstract
Phosphorothioate (PS) modifications are important chemical modifications across oligonucleotide therapeutics. However, the introduction of PS linkages significantly increases analytical complexity due to the generation of diastereomers. Here, we exte…
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