Analytical chemistryHydrophobic and Hydrophilic InteractionsImmunoconjugatesChromatographyLiquidHigh-Throughput Screening Assays
Real-Time Bioconjugation Reaction Monitoring of Antibody-Drug Conjugates with Multiattribute High-Throughput Hydrophobic Interaction Chromatography.
Lance Cadang, Songman Li, Jenny Wang, Andreas Stumpf, Trevor Kempen, Lulu Dai, Alan Bouskila, Corey W Meadows, Abbie Breton, Lian Yi, Yuchen Fan, Tao Chen, Kelly Zhang, Bingchuan Wei
Published: 202510.1021/acs.analchem.5c03553
Abstract
Hydrophobic interaction chromatography (HIC) is a current gold standard for determining the drug-to-antibody ratio (DAR) and drug load distribution (DLD) in antibody-drug conjugates (ADCs). In this study, we developed several HIC methods using ammoni…
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