Methods (San Diego, Calif.)SARS-CoV-2Nucleic Acid Amplification TechniquesRNAViralCOVID-19
Novel primer-less amplification method for detection of RNA molecules.
Alicia Del Prado, María I Martínez-Jiménez, Luis Blanco, Miguel de Vega
Published: 202510.1016/j.ymeth.2025.10.007
Abstract
As shown here, isothermal and primer-less amplification of specific padlock probes allows direct detection of SARS-CoV2 RNA without needing for a reverse transcription step. This simplified method of Hyperbranched Rolling Circle Amplification (HRCA)…
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